Korunga (Onobrychis viciifolia) Hipokotil Eksplantlarının In Vitro Rejenerasyon Yeteneğinin Belirlenmesi
نویسندگان
چکیده
Bu araştırma Korunga (Onobrychis viciifolia Scop.) Özerbey-03 çeşidine ait hipokotil eksplantlarının BAP (6-Benzil Amino Pürin- sitokinin hormonu) ve İAA (İndol-3-Asetik Asit- oksin içeren besi ortamlarındaki gelişimini araştırmak amacıyla gerçekleştirilmiştir. Araştırmada temel ortamı olarak MS (Murashige and Skkog) kullanılmış olup bu ortama 0.5 mg.l-1 IAA ayrı ilave edilmiş, yine kullanılan ortamına + IAA, 0.1 oranlarında hormonları birlikte edilmiştir. Araştırma sonucunda direk rejenerasyon bakımından hiçbir hormon kullanılmayan yalın ortamının en iyi sonucu (%84) verdiği, kallus oluşumu ise ortamın ortamları toplam değerlendirildiğinde (%100) ortamlarının verdiği belirlenmiştir. Ayrıca kalluslardan sürgün rejenerasyonu sonuç (0.83 adet/kallus) ortamdan elde araştırmanın genel korunga bitkisinde rejenrasyon açısından içermeyen ortamlarının, yüksek konsantrasyonda tercih edilmesi başarı oranını arttıracağı söylenebilir.
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ژورنال
عنوان ژورنال: Ziraat mühendisli?i
سال: 2021
ISSN: ['1301-0891', '2651-4494']
DOI: https://doi.org/10.33724/zm.888511